2017-08-04 14:58:172024-12-19 11:25:32
description
transcriptional repressor of citA
transcriptional repressor of [[gene|citA]]
locus
BSU09430
BSU_09430
proteinLength
308
291
geneLength
924
876
product
transcriptional repressor (LysR family)
transcriptional repressor ([SW|LysR family])
ec
outlinks
bsu
BSU09430
BSU_09430
The protein
Protein family
[SW|LysR family]
[SW|LysR family] (according to UniProt)
Biological materials
Mutant
GP1283 (''[[gene|citA]]''::''aphA3''), available in [SW|Jörg Stülke]'s lab
GP1753 (''[[gene|citR]] [[gene|citA]]''::''aphA3''), the kanamycin resistance cassette can be cut out by introducing the ''cre''-rekombinase into the chromosom of ''B. subtilis'', available in [SW|Jörg Stülke]'s lab
GP1283 Δ(''[[gene|citR]]'')::''aphA3'', available in [SW|Jörg Stülke]'s lab
GP1753 Δ(''[[gene|citR]]-[[gene|citA]]'')::''aphA3'', the resistance cassette can be cut out by introducing the ''cre''-rekombinase into the chromosom of ''B. subtilis'', available in [SW|Jörg Stülke]'s lab
GP2360 Δ(''[[gene|citR]]-[[gene|citA]])''::''erm'', the resistance cassette can be cut out by introducing the ''cre''-rekombinase into the chromosom of ''B. subtilis'', available in [SW|Jörg Stülke]'s lab
BKE09430 (Δ[[gene|citR]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTATTCTCCCTCTGA, downstream forward: _UP4_TAGGAGCAACCAAAACGCCT
BKK09430 (Δ[[gene|citR]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTATTCTCCCTCTGA, downstream forward: _UP4_TAGGAGCAACCAAAACGCCT
Biological materials
Expression vector
pGP2264 (N-terminal His-tag, purification from ''E. coli'', in [SW|pETM-11]), available in [SW|Jörg Stülke]'s lab
The protein
Paralogous protein(s)
[[this]]
The protein
[SW|Domains]
[SW|HTH lysR-type domain] (aa 1-58) (according to UniProt)
Biological materials
Expression vectors
pGP2264 (N-terminal His-tag, purification from ''E. coli'', in [SW|pETM-11]), available in [SW|Jörg Stülke]'s lab
pGP1023 (C-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP574]), available in [SW|Jörg Stülke]'s lab
pGP1029 (N-terminal Strep-tag, purification from ''B. subtilis'', in [SW|pGP380]), available in [SW|Jörg Stülke]'s lab
pGP1030 (C-terminal Strep-tag, purification from ''B. subtilis'', in [SW|pGP382]), available in [SW|Jörg Stülke]'s lab
pGP2260 (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab
pGP2262 (integration into [[gene|ganA]], expression under the control of the xylose-inducible PxylA promoter in ''B. subtilis'', in [SW|pGP888]), available in [SW|Jörg Stülke]'s lab