SubtiBank SubtiBank
Version comparison:

2017-08-04 14:58:172024-12-19 11:25:32

description

transcriptional repressor of citA

transcriptional repressor of [[gene|citA]]

locus

BSU09430

BSU_09430

proteinLength

308

291

geneLength

924

876

product

transcriptional repressor (LysR family)

transcriptional repressor ([SW|LysR family])

ec

outlinks

bsu

BSU09430

BSU_09430

The protein

Protein family

[SW|LysR family]

[SW|LysR family] (according to UniProt)

Biological materials

Mutant

GP1283 (''[[gene|citA]]''::''aphA3''), available in [SW|Jörg Stülke]'s lab

GP1753 (''[[gene|citR]] [[gene|citA]]''::''aphA3''), the kanamycin resistance cassette can be cut out by introducing the ''cre''-rekombinase into the chromosom of ''B. subtilis'', available in [SW|Jörg Stülke]'s lab

GP1283 Δ(''[[gene|citR]]'')::''aphA3'', available in [SW|Jörg Stülke]'s lab

GP1753 Δ(''[[gene|citR]]-[[gene|citA]]'')::''aphA3'', the resistance cassette can be cut out by introducing the ''cre''-rekombinase into the chromosom of ''B. subtilis'', available in [SW|Jörg Stülke]'s lab

GP2360 Δ(''[[gene|citR]]-[[gene|citA]])''::''erm'', the resistance cassette can be cut out by introducing the ''cre''-rekombinase into the chromosom of ''B. subtilis'', available in [SW|Jörg Stülke]'s lab

BKE09430 (Δ[[gene|citR]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTATTCTCCCTCTGA, downstream forward: _UP4_TAGGAGCAACCAAAACGCCT

BKK09430 (Δ[[gene|citR]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTATTCTCCCTCTGA, downstream forward: _UP4_TAGGAGCAACCAAAACGCCT

Biological materials

Expression vector

pGP2264 (N-terminal His-tag, purification from ''E. coli'', in [SW|pETM-11]), available in [SW|Jörg Stülke]'s lab

The protein

Paralogous protein(s)

[[this]]

The protein

[SW|Domains]

[SW|HTH lysR-type domain] (aa 1-58) (according to UniProt)

Biological materials

Expression vectors

pGP2264 (N-terminal His-tag, purification from ''E. coli'', in [SW|pETM-11]), available in [SW|Jörg Stülke]'s lab

pGP1023 (C-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP574]), available in [SW|Jörg Stülke]'s lab

pGP1029 (N-terminal Strep-tag, purification from ''B. subtilis'', in [SW|pGP380]), available in [SW|Jörg Stülke]'s lab

pGP1030 (C-terminal Strep-tag, purification from ''B. subtilis'', in [SW|pGP382]), available in [SW|Jörg Stülke]'s lab

pGP2260 (N-terminal Strep-tag, purification from ''E. coli'', in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab

pGP2262 (integration into [[gene|ganA]], expression under the control of the xylose-inducible PxylA promoter in ''B. subtilis'', in [SW|pGP888]), available in [SW|Jörg Stülke]'s lab